Kennedy Krieger Institute

RESEARCH ASSISTANT II - NEUROSCIENCE LABORATORY - DR. WILSON

US-MD-Baltimore
Department
Neuroscience Research
ID
3143
Pos. Category
Research - Other
707 N Broadway

Overview

JOB ID:  3143

 

the Research Assistant II carries out scientific studies of cellular mechanisms of neuronal injury in the developing brain with Dr. Wilson in the Neuroscience Laboratory. The Research Assistant II is able to read and understand the scientific literature, carry out experiments, plan experiments, analyze data and make presentations to others about the work.  A strong background in neuroscience, biology and chemistry as well as previous lab experience is required.  The ability to grasp the rationale for the methods used and careful attention to details of the protocols are important for success.  A high level of responsibility, flexibility, and independence are required to carry out the diverse duties of this position. 

Responsibilities

  1. Plan experiments that use a neonatal rodent model to evaluate mechanisms of hypoxic-ischemic injury and neuroprotection in the developing brain, consulting with Dr. Wilson.
  2. Carry out experiments in immature rodents, using experimental treatments and microsurgical techniques, post-operative care, behavioral studies and euthanasia, in accordance with ACUC-approved protocols and the Guide for the Care and Use of Experimental Animals.
  3. Characterize the brain response to hypoxic ischemic injury and hypothermic neuroprotection using methods such as histopathology and immunohistochemistry: prepare tissue sections and process according to protocols.
  4. Evaluate changes in mRNA and protein expression after hypoxic-ischemic injuy in brain tissue samples using methods such as RT-PCR, ELISA, or other assays.
  5. Use methods such as flow cytometry to evaluate neuroinflammation after hypoxic-ischemic injury.
  6. Collect data using computerized microscopy and image analysis systems; prepare Excel data tables and analyze with statistics packages such as SPSS.
  7. Implement new experimental protocols based on methods described in scientific literature.
  8. Work cooperatively with others in the laboratory to carry out collaborative research.

Qualifications

EDUCATION:

  • BA/BS in biology, chemistry, molecular biology or a related science is required.

 

EXPERIENCE:

  • Equivalent of two years’ experience in laboratory research.
  • Experience with histological techniques and biochemical assays is strongly preferred.

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